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KMID : 0380020030180020100
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 2 p.100 ~ p.106
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Protein Binding of [S]-perillyl Alcohol in HSA using High-Performance Frontal Analysis
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Song Myung-Suk
Wang Duk-Sun
Koo Yoon-Mo
Row Kyung-Ho
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Abstract
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An on-line frontal analysis HPLC system was developed to determine the unbound concentration of (S)-perillyl alcohol, an potential anti-cancer agent, in human serum albumin (HSA) solution, The analysis was performed on a Develosil 100 Diol 5 (10 cm x 4.6 mm I.D.) high-performance frontal analysis (HPFA) column. Sodium phosphate solution was used as the mobile phase (pH 7.4, ionic strength 0.17) at a flow rate of 1 /min. UV wavelength was set at 205 nm. A injection volume of 600 was chosen to ensure the compound eluted formed a zonal peak with a plateau. By Scatchard analysis, it was found that the binding constant(K) and binding number(n) of (S)-perillyl alcohol to molecular HSA were 2.05 x [], 0.00428, respectively.
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KEYWORD
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HPFA, (S)-perilly1 alcohol, human serum albumin, binding parameter
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